Differential Toxicity of DNA Adducts of Mitomycin C

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Differential Toxicity of DNA Adducts of Mitomycin C

The clinically used antitumor agent mitomycin C (MC) alkylates DNA upon reductive activation, forming six covalent DNA adducts in this process. This paper focuses on differential biological effects of individual adducts in various mammalian cell cultures, observed in the authors' laboratories. Evidence is reviewed that various adducts are capable of inducing different cell death pathways in can...

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Repair of mitomycin C mono- and interstrand cross-linked DNA adducts by UvrABC: a new model

Mitomycin C induces both MC-mono-dG and cross-linked dG-adducts in vivo. Interstrand cross-linked (ICL) dG-MC-dG-DNA adducts can prevent strand separation. In Escherichia coli cells, UvrABC repairs ICL lesions that cause DNA bending. The mechanisms and consequences of NER of ICL dG-MC-dG lesions that do not induce DNA bending remain unclear. Using DNA fragments containing a MC-mono-dG or an ICL...

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[Mitomycin C toxicity in rabbit corneal endothelium].

PURPOSE To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. METHODS The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the righ...

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P 190 Corneal endothelial toxicity of mitomycin C in rabbits

Hsldw S.A.1, Buh~r~~nly J.1, Haswll P.D.1, Bowbr I.C.J.W.2, Bmn A.J.1 1 Depatlmenl of Ophthalmology, University ot Oxford. 2 Department of Bacterioboy. Oxford Regional FMii Heaith Laboratory. Purpose: lntravitreal antibiotic is the mainstay of endophthalmitis treatment. Repeat injections should be based on the knowledege of tissue levels. The purpose of this study is to assess the value of aque...

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Pharmacology of mitomycin C. I. Toxicity and pathologic effects.

The toxicity of mitomycin C has been studied in mice, rats, cats, dogs, and rhesus monkeys. Fatal poisoning was uniformly protracted in all five species. Its features were anorexia, steady losses in weight, emesis (in cats and dogs), diarrhea, dehydration, and delayed deaths. Fevers were common in dogs. The agent had notable hématologieeffects. In dogs these included reticulocytopenia, leukope...

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ژورنال

عنوان ژورنال: Journal of Nucleic Acids

سال: 2010

ISSN: 2090-021X

DOI: 10.4061/2010/698960